Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology
نویسندگان
چکیده
منابع مشابه
Histopathologic evaluation of mast cells in reticular and erosive oral lichen planus using toluidine blue staining
Introduction: Oral Lichen Planus (OLP) is one of the most common mucocutaneous lesion, and its etiologic factors have not been elucidated yet. OLP has two different clinical forms including reticular and erosive. Mast cells function in non-specific immunology is an etiologic factor involved in the formation of lichen planus. This study aimed at determining the pattern of mast cells presence in ...
متن کاملApplication of standard micro-anatomical staining methods to epoxy resin-embedded sections.
Staining of semi-thin sections of osmium-fixed epoxy resin (Epon or Araldite)-embedded tissues with haematoxylin and acid dyes as counterstains (eosin or phloxin) was successfully achieved after intense oxidation using hydrogen peroxide as the oxygen donor. Results are closely similar to those obtained with paraffin sections (haematoxylin and eosin): nuclei stain blue, cytoplasm and extracellul...
متن کاملSTUDIES ON NUCLEIC ACID METACHROMASY II. Metachromatic and Orthochromatic Staining by Toluidine Blue of Nucleic Acids in Tissue Sections
Acrolcin-fixed, polyester wax-cmbcddcd tissue sections showed excellent preservation of light microscopic architecture and, when stained with toluidinc blue, intense color contrast between DNA, which stained orthochromatically, and RNA, which stained metachromatically. This method has practical value for differentiating DNA from RNA in the same section. The color c~)ntrast was impaired by subst...
متن کاملSTUDIES ON NUCLEIC ACID METACHROMASY II. Metachromatic and Orthochromatic Staining by Toluidine Blue of Nucleic Acids in Tissue Sections
Acrolcin-fixed, polyester wax-cmbcddcd tissue sections showed excellent preservation of light microscopic architecture and, when stained with toluidinc blue, intense color contrast between DNA, which stained orthochromatically, and RNA, which stained metachromatically. This method has practical value for differentiating DNA from RNA in the same section. The color c~)ntrast was impaired by subst...
متن کاملSTUDIES ON NUCLEIC ACID METACHROMASY II. Metachromatic and Orthochromatic Staining by Toluidine Blue of Nucleic Acids in Tissue Sections
Acrolcin-fixed, polyester wax-cmbcddcd tissue sections showed excellent preservation of light microscopic architecture and, when stained with toluidinc blue, intense color contrast between DNA, which stained orthochromatically, and RNA, which stained metachromatically. This method has practical value for differentiating DNA from RNA in the same section. The color c~)ntrast was impaired by subst...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2018
ISSN: 1940-087X
DOI: 10.3791/58031